[B+ series fluorescence] University Upright Microscopes 정립 현미경

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University Upright Microscopes

정립 현미경

B+ series fluorescence

86-179_led

 

For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc

The B+ series microscope for fluorescence comes in two versions. Two models are equipped with 4 monochromatic LEDs and two other models are equipped with a 100 W mercury vapor illuminator

The microscopes are supplied with fluorescence filters and dichroic mirrors for excitation in the blue spectral region, green, violet and ultraviolet

b_series_fluor_icons

Highlights

  • Trinocular version
  • WF 10x/20 mm eyepieces
  • Reversed nosepiece for maximum 5 objectives
  • Plan and plan fluarex ioS Din objectives
  • Integrated x-Y mechanical stage
  • Coaxial coarse and fine adjustment with adjustable rack stop
  • Height adjustable abbe condenser with iris diaphragm and filter holder
  • Transmitted leD illumination with rechargeable batteries and integrated power supply
  • epi-fluorescence illumination with 4 leDs or 100 W mercury vapor lamp
  • fluorescence filters and dichroic mirror for blue, green, violet and ultraviolet excitation

Models

00

The Fluarex objectives are suitable for fluorescence excitation in the violet and ultraviolet region 4 NeoLEDTM fluorescence: Blue (EX 460 to 470 nm, EM > 515 nm), Green (EX 520 to 530 nm, EM > 590 nm),
Violet (EX 395 to 415 nm, EM 425 nm) and Ultraviolet (EX 355 to 375 nm, EM > 455 nm) 

 

 

Specifications

EYEPIECE(S)
Wide field WF10x/20 mm eyepieces

HEADS
Trinocular 360° rotatable head with 30° inclined tubes. One tube is equipped with diopter adjustment and the interpupillary distance is adjustable between 48 to 76 mm. Trinocular light split 100:0/0:100 

NOSEPIECE
Nosepiece for maximum 5 objectives and mounted on ball bearings 

OBJECTIVES
• The 86.179-LED and86.178-LED models are supplied with plan 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion infinity corrected IOS DIN objectives 
• The 86.180-LED and 86.181-LED models are supplied with plan fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion infinity corrected IOS DIN objectives. 

The plan fluarex objectives allow observation in violet and ultraviolet excitation
All S40x and S100x oil immersion objectives are spring loaded
All optics have an anti-reflection coating for maximum light throughput and are anti-fungus treated 

FOCUSING
Coaxial coarse and fine adjustments, 200 graduations, 1 μm per graduation, 200 μm per rotation, total travel approximately 23 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control 

STAGE
125 x 135 mm stage with 75 x 35 mm X-Y mechanical stage. With Vernier 0.1 mm scale. Removable slide holder. Stages with heating elements and temperature PID controller are also available

CONDENSER
Height adjustable Abbe condenser N.A. 1.25 with iris diaphragm and filter holder

TRANSMITTED ILLUMINATION
Adjustable 3 W LED illumination with 3 rechargeable batteries. Batteries for cordless operation. All models have an integration 100 – 240 V power supply 

FLUORESCENCE ATTACHMENT
Epi-illumination with 4 LEDs for fluorescence excitation from 460 to 470 nm (blue), 520 to 530 nm (green), 390 to 400 (violet) and 360 to 370 nm (ultraviolet). External 100-240 V power supply. Epi-illumination 100 W mercury vapor lamp. External 110 or 230 V power supply. The fluorescence attachment are equipped with dichroic mirrors and fluorescence filters for blue, green, violet and ultra-violet excitation

PACKAGING
Supplied with power cord, dust cover, a spare fuse, user manual and 5 ml immersion oil for models with S100x objective. Supplied with orange UV protection shield. All packed in a polystyrene box

 

Accessories

86.575
Wide field eyepiece HWF 10x/20 mm
86.573
Wide field eyepiece WF 15x/12 mm
80.882
Wide field eyepiece WF 20x/10 mm
86.574
Micrometer eyepiece HWF10x/18, 10mm/100, with adjustable eye lens
80.839
Eyecup for Ø 28 mm eyepiece. Not foldable, curved model
86.891
Semi plan SMP 4x/0.10 objective. Working distance 23,13 mm
86.893
Semi plan SMP 10x/0.25 objective . Working distance 4,09 mm
86.894
Semi plan SMP 20x/0.40 objective. Working distance 1,97 mm
86.897
Semi plan SMP S40x/0.65 objective. Working distance 0,6 mm
80.899
Achromatic S60x/0.85 objective. Working distance 0,20 mm
86.900
Semi plan SMP S100x/1.25 oil immersion objective. Working distance 0,38 mm
86.511
Plan PL 4x/0.10 objective. Working distance 17,9 mm
86.513
Plan PL 10x/0.25 objective. Working distance 8,8 mm
86.514
Plan PL 20x/0.40 objective. Working distance 8,6 mm
86.517
Plan PL S40x/0.65 objective. Working distance 0,56 mm
86.519
Plan PL S60x/0.85 objective. Working distance 0.25 mm
86.520
Plan PL S100x/1.25 oil immersion objective. Working distance 0,33 mm
86.521
Plan PL S100x/1.25 oil immersion objective with iris diaphragm. Working distance 0,33 mm
86.413
Semi plan phase PH 10x/0.25 objective. Working distance 6,61 mm
86.414
Semi plan phase PH 20x/0.40 objective. Working distance 1,85 mm
86.417
Semi plan phase PH S40x/0.65 objective. Working distance 0,64 mm
86.420
Semi plan phase PH S100x/1.25 oil immersion objective. Working distance 0,19 mm
86.313
Plan phase PLPH 10x/0.25 objective. Working distance 8,8 mm
86.314
Plan phase PLPH 20x/0.40 objective. Working distance 8,6 mm
86.317
Plan phase PLPH S40x/0.65 objective. Working distance 0.65 mm
86.320
Plan phase PLPH S100x/1.25 oil immersion objective. Working distance 0,33 mm
86.550
Plan Fluarex PLF 4x /0,15 infinity corrected IOS objective. Working distance 21,6 mm
86.559
Plan PL 2,5x infinity corrected IOS objective
86.560
Plan PL 4x /0,10 infinity corrected IOS objective. Working distance 19,75 mm
86.562
Plan PL 10x/0.25 infinity corrected IOS objective. Working distance 5 mm
86.564
Plan PL 20x/0.40 infinity corrected IOS objective. Working distance 8,8 mm
86.566
Plan PL S40x/0.65 infinity corrected IOS objective Working distance 0,66 mm
86.567
Plan PL S60x/0.80 infinity corrected IOS objective Working distance 0,26 mm
86.568
Plan PL S100x/1.25 oil immersion infinity corrected IOS objective Working distance 0,36 mm
86.613
Simple phase contrast condenser with PLPH S40x objective and phase plate 40x in slider.
86.615
Zernike phase contrast set for B-series with semi plan phase PH 10/20/S40/S100x objectives
86.617
Zernike phase contrast set for B-series with plan phase PLPH 10/20/S40/S100x objectives
86.820
SLR camera adapter with 2.5x and 4x magnification lenses
86.860
Köhler diaphragm with centring, to be mounted on lamp house . Only suitable for models with halogen illumination
86.889
Spare halogen bulb 6 Volt 20 Watt
86.101
Spare 1W LED module
AE.1110
Stage 76 x 26 mm micrometer slide, 1 mm divided in 100 parts, 10 µm per division
AE.1111
Stage micrometer 2 mm divided in 200 parts, 10 µm per division
AE.5130
Universal Ø 23.2 mm tube adapter with built-in 2x lens for SLR photo camera with APS-C sensor. Needs T2 adapter
AE.5040
T2 ring for Canon EOS SLR digital camera
AE.5025
T2 ring for Nikon D SLR digital camera
AE.5202
Blue filter, 32 mm diameter
AE.5203
Yellow filter, 32 mm diameter
AE.5204
Neutral grey filter, 32 mm diameter
AE.5205
Green filter, 32 mm diameter
AE.5207
Blue filter plexiglass, 32 mm diameter
AE.5227
Glass fuses 1A 250V, 10 pieces
AE.5365
Eyecup for Ø 28 mm eyepieces. Not foldable
DC.1320
C-mount adapter with 1x objective for standard Ø 23.2 mm tubes
DC.1324
C-mount adapter with 0.5x objective for standard Ø 23.2 mm tubes and 1/2” camera sensor
DC.1326
C-mount adapter with 0.35x objective for standard Ø 23.2 mm tubes and 1/3” camera sensor
PB.5155
Microscope slides 76 x 26 mm, ground edges, 50 pieces
PB.5165
Cover glasses 18 x 18 mm, 0,13-0,17 mm, 100 pieces
PB.5168
Cover glasses 22 x 22 mm, 0,13-0,17 mm, 100 pieces
PB.5245
Lens cleaning paper, 100 sheets per pack
PB.5255
Immersion oil, 25 ml. Refraction index n = 1,482
PB.5274
Iso propyl alcohol 99%, 200 ml
PB.5275
Cleaning kit: lens fluid, lint free lens tissue/paper, brush, air blower, cotton swabs
86.172
Dark field stop to be placed in filter holder. Suitable for objectives from 10x up to S40x
AE.5168-B
Heating stage with PID controller up to 50°C. Only supplied with a new B or B+ microscope
86.552
Plan Fluarex PLF 10x/0.35 infinity corrected IOS objective. Working distance 2,9 mm
86.554
Plan Fluarex PLF 20x/0.60 infinity corrected IOS objective. Working distance1,5 mm
86.556
Plan Fluarex PLF S40x/0.70 infinity corrected IOS objective Working distance 0,6 mm
86.558
Plan Fluarex PLF S100x/0,90 oil immersion infinity corrected IOS objective Working distance 0,62 mm

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OPTION

86.178-LED, 86.179-LED, 86.180-LED, 86.181-LED