[Oxion fluorescence] UNIVERSITY Fluorescence microscopes 형광 현미경

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UNIVERSITY Fluorescence microscopes

형광 현미경

Oxion fluorescence

ox-3230

For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc

The Oxion microscopes for fluorescence are equipped with an epi-illumination with a 100 W mercury vapor lamp. The fluorescence attachment have a rail for maximum 4 filter cubes. The FluoLed model is equipped with a 455 nm LED and one filter cube, especially developed for efficient and fast detection of tuberculosis by the Auramine-O fluorochrome. Other FluoLED wavelengths are also available

oxion_fluor_icons

 

Highlights

  • Binocular and trinocular models
  • HWF 10x/22 mm eyepieces
  • Reversed nosepiece for maximum 5 objectives
  • Plan, plan fluarex and plan semi apochromatic fluarex ioS objectives
  • Integrated mechanical stage
  • Coaxial coarse and fine adjustments
  • Height adjustable abbe n.a. 1.25 condenser with iris diaphragm and filter holder
  • 3 W lED transmitted illumination
  • Fluorescence attachment for maximum 4 filter blocks and with 100 W mercury vapor incident illuminati
  • Fluorescence attachment with single filter block and 455 nm (Blue), 560 nm (Green) or 365 nm (UV) in
  • Ergonomic stand and head

Models

2016-11-13-152950

Specifications

EYEPIECE(S)
HWF 10x/22 mm eyepieces. All eyepieces can be secured by an Allen screw

HEADS
Binocular and trinocular Siedentopf type heads are with 30° inclined tubes. Interpupillary distance from 50 to 75 mm. Two ± 5 diopter adjustments. A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)

NOSEPIECE
Revolving and reversed nosepiece for maximum 5 objectives. The fluorescence models with mercury vapor lamp are equipped with an orange UV protection shield 

OBJECTIVES
• Plan 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives 
• Plan fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives
• Plan semi apochromatic fluarex 4x/0.10, 10x/0.25, S40x/0.65 and S100x/1.25 oil immersion IOS objectives

All S40x, S60x and S100x oil immersion objectives are spring loaded. All optics are anti-fungus treated and anti-reflection coated for maximum light throughput 

FOCUSING
Coaxial coarse and fine adjustments, 200 graduations,1 μm per graduation, 200 μm per rotation, total travel approximately 28 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control

STAGE
150 x 140 mm stage with integrated X-Y mechanical stage. A ceramic stage is also available on request

CONDENSER FOR BRIGHTFIELD
Height adjustable Abbe condenser N.A. 1.25 with iris diaphragm. Supplied with a white diffusion filter. Phase contrast rings mounted in slider can be inserted in the condenser for 10x and S40x or 20x and S100x phase contrast objectives. A dark field stop in slider for the 10x, 20x and S40x objectives can also be inserted with magnification indication

CONDENSER FOR FLUORESCENCE
The fluorescence attachment is equipped with an integrated condenser with iris diaphragm and with a field diaphragm

ILLUMINATION FOR BRIGHTFIELD AND PHASE CONTRAST
All models are supplied with a 3 W adjustable NeoLED illumination with built-in 100-240 V power supply. Köhler illumination with field diaphragm 

ILLUMINATION FOR FLUORESCENCE
Fluorescence attachment with 100 W (HBO) mercury vapor incident epi-illumination and rail for maximum 4 filter blocks. Two general purpose filter sets are standard supplied with the attachment: AE.3245 (Blue excitation) and AE.3246 (Green excitation). Two other general purpose filter sets AE.3246 (UV excitation) and AE.3247 (Ultraviolet excitation) are also available. More specific filter sets are available on request

STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS

oxion_fluor_graphics

ILLUMINATION FOR FLUOLED ATTACHMENT
The FluoLed attachment is standard delivered with a 455 nm LED for efficient and fast detection of tuberculosis with Auramine-O fluorochrome. FluoLED attachments are also available with a 560 nm LED and Green filter set or a 365 nm LED together with an Ultraviolet filter set

CONFIGURATIONS
The Oxion microscope is available in many configurations and can be upgraded with a variety of contrast types.

• Do you want to see the standard models?
• Do you want to see the phase contrast models?

• Do you want to see the dark field models?
• Do you want to see the inverso (life science) models?
• Do you want to see the material science models?
• Do you want to see the inverso (material science) models?
• Do you want to see the fluorescence models? 

PACKAGING
Supplied with power cord, dust cover, white filter, user manual and 5 ml immersion oil for models with S100x objective. All packed in a polystyrene box

Accessories

AE.3210
HWF 10x/22 mm eyepiece
AE.3215
HWF 15x/13 mm eyepiece
AE.3223
HWF 10w/22mm eyepiece with 10mm/100 micrometer reticule
AE.3225
Pair of eyecups
AE.3160
Infinity semi plan SMP 4x/0.10 IOS objective. Working distance 21 mm
AE.3162
Infinity semi plan SMP 10x/0.25 IOS objective . Working distance 5,95 mm
AE.3166
Infinity semi plan SMP S40x/0.65 IOS objective. Working distance 0,64 mm
AE.3270
Infinity semi plan SMP S100x/1.25 oil immersion IOS objective. Working distance 0,36 mm
AE.3102
Infinity plan PL 2x/0,06 IOS objective
AE.3104
Infinity plan PL 4x/0.10 IOS objective. Working distance 11,9 mm
AE.3106
Infinity plan PL 10x/0.25 IOS objective. Working distance 12,1 mm
AE.3108
Infinity plan PL 20x/0.40 IOS objective. Working distance 1,5 mm
AE.3110
Infinity plan PL S40x/0.65 IOS objective. Working distance 0,36 mm
AE.3112
Infinity plan PL S60x/0.85 IOS objective. Working distance 0,3 mm
AE.3114
Infinity plan PL S100x/1.25 oil immersion IOS objective. Working distance 0,18 mm
86.550
Plan Fluarex PLF 4x /0,15 infinity corrected IOS objective. Working distance 21,6 mm
AE.3144
Plan Fluarex semi apochromatic PLF 4x/0,13 infinity corrected IOS objective
AE.3146
Plan Fluarex semi apochromatic PLF 10x/0,30 infinity corrected IOS objective
AE.3148
Plan Fluarex semi apochromatic PLF 20x/0,50 infinity corrected IOS objective
AE.3152
Plan Fluarex semi apochromatic PLF S40x/0,75 infinity corrected IOS objective
AE.3156
Plan Fluarex semi apochromatic PLF S100x /1,28 oil immersion infinity corrected IOS objective Working distance 0,21 mm
AE.3240
Fluorescence illuminator with 100 Watt mercury vapor lamp, inclusive power supply unit and UV protection shield
AE.3245
Fluorescence block with general purpose filter set for Blue EX475BP40, DM505LP,EM535BP45. Excellent for FITC, eGFP. Compatible for 5-carboxyfluorescein (5-FAM), Alexa fluor 488, BODIP-FL, Calcein, Cy2, Dio (Dio 18(3)), eGFP, FITC, Fluo-4, Fluor-Jade, FluorX, iLyte Fluor 488, Mito tracker Green, Rhodamine 110, Spectrumgreen 110, SYTO-13, YO-PRO-1, YOYO-1
AE.3246
Fluorescence block with general purpose filter set for Green EM560BP55, DM595LP, EM645BP75. Excellent for Texas Red, Alexa 594, 5-Carboxynaphthofluorescein. Other compatible fluorophores: 5-CNF, 5-ROX (carboxy-X-rhodamine),Alexa Fluor 568, Alexa Fluor 594, BOBO-3, BODIPY-TR-X, BO-PRO-3, Calcium Crimson, Cy3.5, HcRed, HiLyte Fluor 594, Lissamine rhodamine B, Mito Tracker Red, mPlum mRaspberry, resorufin, Rhodamine Red, R-phycocyanin, SpectrumRed, Texas Red, X-Rhodaline (XRITC), YO-PRO-3, YOYO-3
AE.3247
Fluorescence block with general purpose filter set for Violet EX440BP21, DM455LP, EM480BP30. Compatible fluorophores: BOBO-1, BOPRO-1, Cerulean, CFP, POPO-1, PO-PRO-1, SpectrumAqua, SYTO45, SYTOX Blue
AE.3248
Fluorescence block with general purpose filter set for UV EX365BP50, DM400LP, EM450BP40. Compatible fluorophores: 4-Methylumbelliverone, 7-amino-4methylcoumarin, 7-Hydrixy-4-methylcoumarin (pH9), Alexa Fluor 350, AMCA, AMCA-X, Calcein Blue, Calcein White, Cascade Blue, DAPI, Hoechst 33342 & 33258, LysoSensor Blue (pH5), Lyso Tracker Red, marina Blue, Spectrum Blue
OX.9750.0401
Premium fluorescence filter set EX440BP21 DM455LP EM480BP30 optimized for CFP. Compatible with eCFP, mCFPm, Cerulean, CyPet adn SpectrumAqua. This filter set is designed for optimal signal capture of CFP and to minimize spectral bleed-through of YFP. Needs empty block OX.9749
OX.9750.0403
Premium fluorescence filter set EX380BP50 DM410LP EM450BP60 optimized for BFP, BFP2. Compatible with AlexaFluor 350, DAPI; Hoeschst 33342 & 33258. Wide excitation filter bandwidth may cause cellular damage in live cell applications. Needs empty block OX.9749
OX.9750.0404
Premium fluorescence filter set EX470BP40 DM500LP EM535BP50 optimized for AlexaFluor 488. Compatible with Cy2, eCFP, FITC and GPT (sapphire). Designed for both excellent brightness and contrast with greater than OD 6 at the excitation/emission crossover. Needs empty block OX.9749
OX.9750.0407
Premium fluorescence filter set EX635BP30 DM660LP EM710BP80 optimized for AlexaFluor 647. Compatible with Cy5, TOTO-1, TOTO-3, Kodak X-Sight 650. A wide emission filter for maximum photon capture. A narrow excitation filter. Needs empty block OX.9749
OX.9750.0412
Premium fluorescence filter set EX500BP25 DM525LP EM545BP35 optimized for YFP and for minimizing CFP bleedthrough. Compatible with AlexFluor 532, Acridine Orange (+DNA), Rhodamine Green . Needs empty block OX.9749
OX.9750.0414
Premium fluorescence filter set EX560BP55 DM595LP EM645BP75 optimized for Texas Red. Compatible with Texas Red-X, Mito Tracker Red, Cy 3,5, HcRed, mRasberry. Wide passbands that give high brightness and contrast to red emitting fluorophores such as Texas Red. Needs empty block OX.9749
OX.9750.39001
Fluorescence filter set EX435 (BP20) DM455 (LP) EM480 (BP30) optimzed for AmCyan, Atto 425, Cerulean, CFP, ECFP fluorophore. Needs empty filter block (OX.9749)
OX.9750.39002
Fluorescence filter set EX480 (BP30) DM505(LP) EM535 (BP40) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block (OX.9749)
OX.9750.39003
Fluorescence filter set EX495 (BP20) DM515 (LP) EM540 (BP30) optimized for Alexa Fluor 514™, Citrine, EYFP/pH 7, F/lAsH-CCPFCC, mCitrine, Oregon Green™ 514, Rhodamine 123, Topaz. Needs empty filter block (OX.9749)
OX.9750.39004
Fluorescence filter set EX540 (BP25) DM565 (LP) EM605 (BP55) optimized for Alexa Fluor 546™, Alexa Fluor 555™, Atto 550, CAL, Fluor® Red 590, Cy3™, DiI, DsRed, DyLight 549, MitoTracker Orange/MeOH, Resorufin, Rhod-2, TagRFP, TAMRA tdTomato, Tetramethylrhodamine, isothiocyanate, TRITC. Needs empty filter block (OX.9749)
OX.9750.39005
Fluorescence filter set EX540 (BP25) DM565 (LP) EM 620 (BP60) optimized TRITC-red-shifted, Ethidium homidimer-1/DNA, Propidium Iodide. Needs empty filter block (OX.9749)
OX.9750.39007
Fluorescence filter EX620 (BP50) DM655 (LP) EM 690 (BP50) optimized for Alexa Fluor 647™, Allophycocyanin (APC), Atto 647N, Cy5™, DiD, Draq5, DyLight 649 MitoTracker Deep Red 633/MeOH, Nile Blue, Quasar® 670, SYTO® 60, TO-PRO™-3. Needs empty filter block (OX.9749)
OX.9750.39008
Fluorescence filter set EX420 (BP40) DM455 (LP) EM 535 (BP40) optimized for Lucifer Yellow/Tetracycline/AlexaFluor 430. Needs empty filter block (OX.9749)
OX.9750.39009
Fluorescence filter set EX480 (BP30) DM505 (LP) EM 620 (BP60) for Acridine Orange + RNA, Di-8-ANEPPS, non-ratiometric, DiA, Ethidium Bromide, FM™ 1-43, LysoTracker Yellow HCK-123, Pulsar™ 650. Needs empty filter block (OX.9749)
AE.5120
Standard 23,2 mm tube for Oxion upright (revision 2) and Oxion Inverso inverted microscopes
AE.5130
Universal Ø 23.2 mm tube adapter with built-in 2x lens for SLR photo camera with APS-C sensor. Needs T2 adapter
AE.5025
T2 ring for Nikon D SLR digital camera
AE.5040
T2 ring for Canon EOS SLR digital camera
AE.5057
C-mount adapter 0,5x for 1/2 inch cameras for Oxion (revision 1)
AE.5058
C-mount adapter 0,33x adapter for 1/3 inch camera for Oxion (revision 1)
OX.9833
Photo port adapter with 0,33x lens for Oxion (revision 2) and Oxion Inverso microscopes and 1/3 inch camera with C-mount
OX.9850
Photo port adapter with 0,5x lens for Oxion (revision 2) and Oxion Inverso microscopes and 1/2 inch camera with C-mount
AE.1385
100 W HBO mercury lamp for microscopes with fluorescence attachment
AE.3199
Glass fuses 3.15A 250V, 10pcs
SL.5511
3W NeoLED™ replacement for Oxion
PB.5155
Microscope slides 76 x 26 mm, ground edges, 50 pieces
PB.5165
Cover glasses 18 x 18 mm, 0,13-0,17 mm, 100 pieces
PB.5255
Immersion oil, 25 ml. Refraction index n = 1,482
PB.5274
Iso propyl alcohol 99%, 200 ml
PB.5275
Cleaning kit: lens fluid, lint free lens tissue/paper, brush, air blower, cotton swabs

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추가 정보

OPTION

OX.3067, OX.3070, OX.3075, OX.3080, OX.3085, OX.3230, OX.3232, OX.3235, OX.3237